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Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution transductants. When a chromosomal lac gene was the recipient DNA substrate molecule, both the normalized transduction frequency and the proportion of addition and substitution transductants showed essentially no recB dependence. There was a pronounced recB dependence for both normalized transduction frequency and recombination end product formation when F42 lac was the recipient DNA substrate. recB appears to have no significant role in the recombination that occurs between the two lac regions in an addition transductant. UV irradiation of the transducing phages increased the absolute level of both addition and substitution transductants obtained with a chromosomal lac gene but resulted in a considerable change in the relative frequency of addition versus substitution transductants.
R D Porter, and
R A Welliver, and
T A Witkowski
May 1981,
Journal of virology,
R D Porter, and
R A Welliver, and
T A Witkowski
October 1983,
Genetics,
R D Porter, and
R A Welliver, and
T A Witkowski
January 1981,
Molecular & general genetics : MGG,
R D Porter, and
R A Welliver, and
T A Witkowski
March 1976,
Virology,
R D Porter, and
R A Welliver, and
T A Witkowski
February 1984,
Nucleic acids research,
R D Porter, and
R A Welliver, and
T A Witkowski
September 1984,
Nucleic acids research,
R D Porter, and
R A Welliver, and
T A Witkowski
July 1970,
Virology,
R D Porter, and
R A Welliver, and
T A Witkowski
May 1986,
Bioorganicheskaia khimiia,
R D Porter, and
R A Welliver, and
T A Witkowski
February 1988,
Virology,
R D Porter, and
R A Welliver, and
T A Witkowski
May 1983,
Bioorganicheskaia khimiia,
