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To obtain a placental microsome preparation able to convert androstenedione and testosterone specifically to estrogens, 3 beta-hydroxysteroid dehydrogenase must be eliminated. After solubilisation by Triton X-100, the remaining 3 beta-hydroxysteroid dehydrogenase activity is inhibited by 11 alpha-bromoacetoxyprogesterone, an alkylating analogue of progesterone, which behaves as an irreversible active site-directed inhibitor. The enzyme is protected against inactivation and alkylation by steroid and coenzyme substrates. The inhibition is specific to 3 beta-hydroxysteroid dehydrogenase and the estrogen synthetase (aromatase) activity contained in the preparation is not affected by this inhibitor.
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
November 1994,
The Biochemical journal,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
January 1997,
Biochemistry,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
October 1978,
Biochemistry,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
January 1969,
Bulletin de la Societe de chimie biologique,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
March 1982,
The Journal of biological chemistry,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
December 1986,
The Biochemical journal,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
August 1992,
The Journal of biological chemistry,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
February 1965,
The Journal of endocrinology,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
January 1980,
Steroids,
Y Chikhaoui, and
A M Boussioux, and
J C Nicolas, and
B Descomps, and
A Crastes de Paulet
February 1967,
Nihon Sanka Fujinka Gakkai zasshi,
