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The role of lipid peroxidation (LPO) in the damages of the enzymic system of Ca2+ transport in sarcoplasmic reticulum (SR) membranes of skeletal and cardiac muscles under conditions of vitamin E deficiency, ischemia and limb reoxygenation as well as in emotional-pain stress was investigated. It was shown that these processes are associated with activation of endogenous LPO in SR membranes "in vivo" and with simultaneous inhibition of Ca2+ transport, (i. e. decrease of the Ca2+/ATP ratio) and inactivation of Ca-ATPase. The degree of damage of the Ca2+ transport system was correlated with the concentration of LPO products accumulated in SR membranes "in vivo and during LPO induction by the Fe2+ + ascorbate system 'in vitro". Injection of natural and synthetic free radical scavengers (e. g. 4-methyl-2.6-ditretbutylphenol, alpha-tocopherol) to experimental animals resulted in practically complete suppression of LPO activation "in vivo" and in partial protection of the Ca2+-transporting capacity of SR membranes. A comparison of experimental results allowed to estimate the role of LPO in SR damage under pathological conditions. Model experiments with "contraction-relaxation" cycles including isolated components of muscle fibers (SR fragments and myofibrils) demonstrated that LPO induction in SR membranes by the Fe2+ + ascorbate system results in complete elimination of the relaxation step in myofibrils due to the loss of the SR affinity to decrease the concentration of Ca2+ in the incubation medium. This effect can be removed by free radical scavengers. The role of LPO in pathological changes of muscle contractility is discussed.
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
May 1983,
Biokhimiia (Moscow, Russia),
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
January 1986,
Ukrainskii biokhimicheskii zhurnal (1978),
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
November 1986,
Biulleten' eksperimental'noi biologii i meditsiny,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
January 1980,
Doklady Akademii nauk SSSR,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
September 1988,
Biulleten' eksperimental'noi biologii i meditsiny,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
May 1983,
Biochimica et biophysica acta,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
January 1982,
Annals of the New York Academy of Sciences,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
November 1981,
Biulleten' eksperimental'noi biologii i meditsiny,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
January 1997,
Biochimica et biophysica acta,
V E Kagan, and
Iu V Arkhipenko, and
F Z Meerson, and
Iu P Kozlov
January 1977,
Advances in experimental medicine and biology,
