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RNA synthesis in Escherichia coli was immediately inhibited after addition of seminal plasmin, an antimicrobial protein from bull semen. RNA synthesis progressively decreased within 12 min and then ceased completely. In contrast, protein synthesis was not affected within the first 12 min, but thereafter became progressively inhibited. Inhibition of RNA synthesis by seminal plasmin in E. coli interfered with induction of beta-galactosidase by isopropyl-beta-D-thiogalactoside (IPTG). This implied inhibition of beta-galactosidase mRNA synthesis by seminal plasmin in vivo. The sensitivities of total in vivo RNA synthesis and beta-galactosidase mRNA synthesis against seminal plasmin were found to be similar. Seminal plasmin had no effect on the uptake of the inducer IPTG by E. coli cells.
K H Scheit, and
M Zimmer
November 1990,
FEMS microbiology letters,
K H Scheit, and
M Zimmer
January 1983,
The EMBO journal,
K H Scheit, and
M Zimmer
August 1983,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
K H Scheit, and
M Zimmer
February 1985,
Journal of biochemistry,
K H Scheit, and
M Zimmer
February 1994,
Preparative biochemistry,
K H Scheit, and
M Zimmer
June 1979,
Nature,
K H Scheit, and
M Zimmer
May 1987,
Biological chemistry Hoppe-Seyler,
K H Scheit, and
M Zimmer
June 1992,
FEBS letters,
K H Scheit, and
M Zimmer
September 1993,
FEMS microbiology letters,
K H Scheit, and
M Zimmer
July 1982,
Nature,
