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Alkanoyl-N-methylglucamides, nonionic detergents, were utilized to solubilize membrane proteins of Escherichia coli and were used to reconstitute them into liposomes. First, critical micelle concentrations (CMC) of nonanoyl-N-methylglucamide and decanoyl-N-methylglucamide were determined to be 25 mM and 7 mM, respectively, by photometric assay. Then solubilization and reconstitution of the melibiose transport carrier were performed using these detergents at concentrations above the CMC. Melibiose counterflow activity was observed with the proteoliposomes reconstituted from the extracted proteins and phospholipids. The proton-translocating ATPase complex (F1-F0) was also solubilized with these detergents. These results indicate that nonanoyl- and decanoyl-N-methylglucamide are useful detergents for solubilization and reconstitution of membrane proteins.
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
June 1982,
FEBS letters,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
August 1977,
FEBS letters,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
August 1985,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
January 1991,
Methods in cell biology,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
November 1980,
The Journal of biological chemistry,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
February 1986,
The Journal of biological chemistry,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
April 1990,
Proceedings of the National Academy of Sciences of the United States of America,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
March 1990,
The Journal of biological chemistry,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
February 1997,
European journal of biochemistry,
M Hanatani, and
K Nishifuji, and
M Futai, and
T Tsuchiya
April 1973,
Biochimica et biophysica acta,
