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Two monoclonal antibodies--one that blocks ristocetin-induced platelet binding of von Willebrand factor to glycoprotein Ib and one that blocks adenosine diphosphate-induced binding of fibrinogen to the glycoprotein IIb/IIIa complex--were used to assess the binding site(s) for von Willebrand factor when platelets are stimulated with thrombin or adenosine diphosphate (ADP). Neither agonist induced binding of von Willebrand factor to glycoprotein Ib. ADP and thrombin induced von Willebrand factor binding exclusively to the glycoprotein IIb/IIIa complex. The results of the site of binding of von Willebrand factor with thrombasthenic platelets were consistent with the data obtained with the monoclonal antibodies and normal platelets. Human fibrinogen caused complete inhibition of thrombin-induced von Willebrand factor binding to normal platelets at concentrations considerably below that found in normal plasma. We conclude that thrombin induces very little binding of exogenous von Willebrand factor to platelets at normal plasma fibrinogen levels.
H R Gralnick, and
S B Williams, and
B S Coller
January 1992,
Methods in enzymology,
H R Gralnick, and
S B Williams, and
B S Coller
February 1990,
Blood,
H R Gralnick, and
S B Williams, and
B S Coller
April 1985,
The Journal of clinical investigation,
H R Gralnick, and
S B Williams, and
B S Coller
November 1985,
Proceedings of the National Academy of Sciences of the United States of America,
H R Gralnick, and
S B Williams, and
B S Coller
January 1989,
Biochemistry,
H R Gralnick, and
S B Williams, and
B S Coller
September 1985,
Blood,
H R Gralnick, and
S B Williams, and
B S Coller
March 1992,
Biochimica et biophysica acta,
H R Gralnick, and
S B Williams, and
B S Coller
November 1985,
The Journal of clinical investigation,
H R Gralnick, and
S B Williams, and
B S Coller
October 1982,
Proceedings of the National Academy of Sciences of the United States of America,
H R Gralnick, and
S B Williams, and
B S Coller
July 1999,
Thrombosis and haemostasis,
