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Six new Marek's disease (MD) lymphoblastoid cell lines were established in vitro by cultivation in a medium containing 2-mercaptoethanol (2-ME). Attempts using primary lymphoma cells were generally unsuccessful; only one of 28 lymphomas yielded a cell line and that one came from an experimentally immunosuppressed chicken. In contrast, two of seven low-passage, and two of two established MD transplantable lymphomas grew readily in vitro. A sixth line was obtained using buffy coat cells from a leukemic chicken. It was concluded that the use of transplantable tumor cells and a medium containing 2-ME provided a combination highly suited to the establishment of cell lines from MD.
B W Calnek, and
K K Murthy, and
K A Schat
September 1983,
Poultry science,
B W Calnek, and
K K Murthy, and
K A Schat
January 1975,
IARC scientific publications,
B W Calnek, and
K K Murthy, and
K A Schat
September 1974,
Nature,
B W Calnek, and
K K Murthy, and
K A Schat
March 1977,
Biken journal,
B W Calnek, and
K K Murthy, and
K A Schat
January 1982,
International journal of cancer,
B W Calnek, and
K K Murthy, and
K A Schat
March 1979,
Biken journal,
B W Calnek, and
K K Murthy, and
K A Schat
September 1974,
Biken journal,
B W Calnek, and
K K Murthy, and
K A Schat
January 1975,
IARC scientific publications,
B W Calnek, and
K K Murthy, and
K A Schat
January 1984,
Avian diseases,
B W Calnek, and
K K Murthy, and
K A Schat
December 1990,
Nihon juigaku zasshi. The Japanese journal of veterinary science,
