Biomaterial Database

Changes in Leydig cells and luteinizing hormone receptors in porcine testis during postnatal development. 1981

J P Peyrat, and N Meusy-Dessolle, and J Garnier

LH receptors have been characterized in porcine testis (Sus crofa L.) from birth to 220 days of age and have been related to interstitial tissue development (especially Leydig cells). The mean association constant (Ka) of ovine [3H]LH, was 7 +/- 6 X 10(9) M-1, with no apparent age-dependent variation but with some significant individual variations. The concentration of specific LH receptor sites reached a maximum of 9 X 10(-12) M/g testis between days 20-70, decreased to 3 X 10(-12) M/g testis at the onset of puberty (up to 100 days), and remained stable at the same level in the adult. The total number of sites per testis essentially reflected the growth of the testis. Interstitial tissue occupied up to 80% of the volume of the whole testis during the occupied up to 80% of the volume of the whole testis during the first 30 neonatal days. It decreased to about 25% after 120 days. The Leydig cells in this tissue occupied the same proportion (70% of the volume of the whole testis) regardless of the age of the animals. The mean Leydig cell diameter reached a maximum of 20 micrometer at 30 days of neonatal life, decreased to a minimum of 10 micrometer at 90 days, and then increased to a stable value of 15 micrometer after puberty. The number of Leydig cells per unit volume varied with age, with a maximum of 3 X 10(8) cells/ml testis at 90 days, reaching a constant value of 6 X 10(7) cells/ml testis after puberty. These data suggest that each Leydig cell contains 80,000 specific LH-binding sites/cell 30 days after birth and 35,000 in the adult, with no observed periods without receptor sites. The number of receptors per cell is correlated to cell size rather than stage of sexual maturation. (Endocrinology 108: 625, 1981)

UI	MeSH Term	Description	Entries
D007985	Leydig Cells	Steroid-producing cells in the interstitial tissue of the TESTIS. They are under the regulation of PITUITARY HORMONES; LUTEINIZING HORMONE; or interstitial cell-stimulating hormone. TESTOSTERONE is the major androgen (ANDROGENS) produced.	Interstitial Cells, Testicular,Leydig Cell,Testicular Interstitial Cell,Testicular Interstitial Cells,Cell, Leydig,Cell, Testicular Interstitial,Cells, Leydig,Cells, Testicular Interstitial,Interstitial Cell, Testicular
D007986	Luteinizing Hormone	A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.	ICSH (Interstitial Cell Stimulating Hormone),Interstitial Cell-Stimulating Hormone,LH (Luteinizing Hormone),Lutropin,Luteoziman,Luteozyman,Hormone, Interstitial Cell-Stimulating,Hormone, Luteinizing,Interstitial Cell Stimulating Hormone
D008297	Male		Males
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012741	Sexual Maturation	Achievement of full sexual capacity in animals and in humans.	Sex Maturation,Maturation, Sex,Maturation, Sexual
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013737	Testis	The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.	Testicles,Testes,Testicle
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In