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[Molecular weight determination of nuclear proteins by disc-gradient polyacrylamide gel electrophoresis]. 1978

S D Kaz'min, and S D Sherban

A technique of molecular weight estimation in proteins according to their electrophoretic mobility in the gradient polyacrylamide gel is used. The method permits analysing the highly heterogenic protein mixtures and determining the molecular weight of proteins within the range of 5000--400.000 simultaneously. The data of the protein spectrum analysis of the liver cell nuclei and some tumors are presented.

UI MeSH Term Description Entries
D008433 Mathematics The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Mathematic
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009698 Nucleoproteins Proteins conjugated with nucleic acids. Nucleoprotein
D004589 Electrophoresis, Disc Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones. Electrophoresis, Disk,Disc Electrophoresis,Disk Electrophoresis

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