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Late IgG and IgM from a rabbit immunized with herpes virus were tested for ordinary neutralizing (N) antibody, complement-requiring neutralizing (CRN) antibody and in addition CRN antibody detectable by overnight sensitization at 0 C (s-CRN antibody). Heat stability tests showed that IgG s-CRN antibody was slightly less resistant to heating at 70 C than were N and CRN antibodies, whereas all three activities of IgM were quickly degraded at this temperature. Dose-response curves with varying amounts of complement (C) or anti-antibody revealed a marked difference between IgG s-CRN and IgM s-CRN antibodies. While 1-hr sensitization at 37 C was insufficient to detect IgG s-CRN antibody, it had the same effect as overnight sensitization at 0 C for IgM s-CRN antibody. When sensitization at 0 C was prolonged to 3 days, unexpectedly high endpoints exceeding 1:10,000 were obtained even with IgM. consequently, enhancement by C was several hundred-fold with IgM in contrast to 5- to 10-fold enhancement of IgG s-CRN antibody, which was similar to that after overnight sensitization. Also IgM obviously required more C than did IgG. These results suggest that IgM of late serum is slower reacting and more C-dependent than IgG s-CRN antibody. Tests with early rabbit sera indicated that the s-CRN antibody detectable by 3-day sensitization reaches a high level before the appearance of N antibody.
K Yoshino, and
M Urayama, and
K Abe
June 1983,
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K Yoshino, and
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K Yoshino, and
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January 1982,
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K Yoshino, and
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K Abe
July 1982,
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K Yoshino, and
M Urayama, and
K Abe
May 1965,
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K Yoshino, and
M Urayama, and
K Abe
February 1964,
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K Yoshino, and
M Urayama, and
K Abe
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K Yoshino, and
M Urayama, and
K Abe
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K Yoshino, and
M Urayama, and
K Abe
January 1981,
Microbiology and immunology,
K Yoshino, and
M Urayama, and
K Abe
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Medical microbiology and immunology,
