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A simplified procedure for purification of nuclease from Serratia marcescens, including chromatography on DEAE- and phosphocellulose in a stationary regime has been developed. The method described results in a physically homogenous enzyme, which does not contain phosphatase, phosphodiesterase or proteinase admixtures. The molecular weight of the enzyme as determined by polyacrylamide gel electrophoresis is 33 000 +/- 10%. p-Chloromercurybenzoate (10(-2) M) completely inactivates the enzyme, while beta-mercaptoethanol (0,64 M) in the presence of 2 M urea causes only partial inactivation of the enzyme. Urea (4 or 7 M), when added to the reaction mixture, increases the enzyme activity 2,2-, 1,7- and 1,4-fold as compared to native, denaturated DNA and RNA, respectively.
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
February 1963,
Journal of bacteriology,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
March 1991,
Biokhimiia (Moscow, Russia),
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
January 1979,
Molekuliarnaia biologiia,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
August 1998,
FEMS microbiology letters,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
April 1998,
Journal of bacteriology,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
October 1969,
The Journal of biological chemistry,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
February 1969,
Nihon saikingaku zasshi. Japanese journal of bacteriology,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
June 1996,
Gene,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
January 1996,
Biochimica et biophysica acta,
M N Filimonova, and
N P Balaban, and
F P Sharipova, and
I B Leshchinskaia
October 1969,
The Journal of biological chemistry,
