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To provide an in vitro system for studies of brain capillary function we developed a method for culture of brain capillary endothelial cells. Capillaries were isolated from rat brain and enzymatically treated to remove the basement membrane and contaminating pericytes. Subsequent Percoll gradient centrifugation resulted in a homogeneous population of capillary endothelial cells that attached to a collagen substrate and incorporated [3H]thymidine. Evidence for the endothelial nature of these cells was provided by the presence of Factor VIII antigen and angiotensin converting enzyme activity and by the failure of platelets to adhere to the cell surface. In addition, the cells were joined together by tight junctions. Thus, primary cultures of these cells retained both endothelial and blood-brain barrier features.
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
January 1977,
Cell and tissue research,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
August 1990,
Molecular and cellular neurosciences,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
November 1975,
Microvascular research,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
July 1985,
Federation proceedings,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
October 1994,
Brain research,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
January 1981,
The Italian journal of biochemistry,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
August 2015,
Fluids and barriers of the CNS,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
March 1985,
In vitro cellular & developmental biology : journal of the Tissue Culture Association,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
March 1997,
Neuroscience letters,
P D Bowman, and
A L Betz, and
D Ar, and
J S Wolinsky, and
J B Penney, and
R R Shivers, and
G W Goldstein
February 2004,
Journal of neurochemistry,
