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Two continuous cell lines (TB-M and TB-6c) derived from epithelial cells of the toad urinary bladder form epithelia in culture that manifest hormone-sensitive transepithelial transport. Development of transepithelial electrical resistance (R) and transport rate (ISC) are dependent on time and density of cells seeded, but steady-state ISC and R are characteristic for each cell line and independent of seeding density. Some responses of intact toad bladder are preserved in culture, whereas others are altered or absent. Neither cell line responds to vasopressin. Analogues of cAMP increase sodium transport and urea permeability in both cell lines but do not affect water permeability. The intramembrane particle aggregates associated with the vasopressin- and cAMP-induced increase in water permeability of the intact bladder could not be detected in the cell lines. Aldosterone increases sodium transport in both cell lines, and the time course and concentration dependence of the response to aldosterone are similar to those of the intact bladder. The relative effect of a series of steroids on ISC reveals corticosterone to be a more potent mineralocorticoid in cultured cells than in the intact bladder.
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
January 1975,
The Journal of membrane biology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
June 1960,
Experimental cell research,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
June 1971,
The Journal of membrane biology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
June 1991,
The American journal of physiology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
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J S Handler
January 1987,
The Journal of membrane biology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
January 1975,
Physiological chemistry and physics,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
February 1980,
The American journal of physiology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
July 1979,
The Journal of membrane biology,
J P Johnson, and
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J B Wade, and
A S Preston, and
S W Green, and
J S Handler
April 1994,
The American journal of physiology,
J P Johnson, and
R E Steele, and
F M Perkins, and
J B Wade, and
A S Preston, and
S W Green, and
J S Handler
September 1970,
Proceedings of the National Academy of Sciences of the United States of America,
