Biomaterial Database

Sensitive radioimmunological screening test for antithyroglobulin autoantibodies. 1978

P Bodlaender, and J R Arjonilla, and S L Twomey

The test described here was designed as a screening test to be used in conjunction with the radioimmunoassay for human thyroglobulin. Anti-thyroglobulin autoantibodies, when present even in low concentrations, interfere with thyroglobulin determination. A 30-min incubation allows binding of 125I-thyroglobulin to endogenous anti-thyroglobulin autoantibodies. A subsequent 2-h incubation with goat anti-human gamma-globulin causes precipitation of such complexes. The immunoprecipitable radioactivity reflects the binding capacity of the sample for 125I-labeled thyroglobulin. Samples that are devoid of autoantibodies, and are therefore suitable for valid thyroglobulin determinations, bind less than 6% of the radiolabeled thyroglobulin. The sensitivity of this radioimmunoassay exceeds that of the tanned-erythrocyte hemagglutination test.

UI	MeSH Term	Description	Entries
D007457	Iodine Radioisotopes	Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.	Radioisotopes, Iodine
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D001798	Blood Proteins	Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.	Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D006386	Hemagglutination Tests	Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)	Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001323	Autoantibodies	Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.	Autoantibody
D013954	Thyroglobulin
D013959	Thyroid Diseases	Pathological processes involving the THYROID GLAND.	Disease, Thyroid,Diseases, Thyroid,Thyroid Disease