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A clonal hamster beta cell line (HIT) was established by simian virus 40 transformation of Syrian hamster pancreatic islet cells. Cytoplasmic insulin was detected in all cells by indirect fluorescent antibody staining, and membrane-bound secretory granules were observed ultrastructurally. Acidified-ethanol extracts of HIT cell cultures contained hamster insulin as determined by radioimmunoassay, radioreceptor assay, and bioassay. One subclone at passage 39 contained 2.6 micrograms of insulin per mg of cell protein. [3H]Leucine-labeled HIT insulin and proinsulin were identical to islet-derived proteins when compared by NaDodSO4/polyacrylamide gel electrophoresis of immunoprecipitates. HIT cell insulin secretion was stimulated by glucose, glucagon, and 3-isobutyl-1-methylxanthine. Insulin secretion at optimal glucose concentration (7.5 mM) was 2.4 milliunits per 10(6) cells per hr. Somatostatin and dexamethasone markedly inhibited HIT insulin secretion. The HIT cell line represents a unique in vitro system for studying beta cell metabolism and insulin biosynthesis.
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
October 1986,
Diabetologia,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
April 1989,
Endocrinology,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
March 1987,
FEBS letters,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
February 1985,
Diabetes,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
November 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
July 1973,
Journal of the National Cancer Institute,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
September 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
September 1980,
Journal of the National Cancer Institute,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
February 1988,
Gastroenterologia Japonica,
R F Santerre, and
R A Cook, and
R M Crisel, and
J D Sharp, and
R J Schmidt, and
D C Williams, and
C P Wilson
May 1987,
Journal of virology,
