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We present an improved method for ultraviolet spectrophotometry of theophylline in serum. We studied various extraction techniques aimed at eliminating interferences from co-extractable serum constituents. In the resulting modified procedure, 1 ml of serum is required and a salt-solvent pair of ammonium sulfate and chloroform/hexane is used for extraction. The solvent forms the top phase after extraction, the lower phase after back-extraction, thereby permitting easy removal of the appropriate phase from culture tubes. The use of ammonium sulfate coupled with the added specificity of the extraction solvent results in an extract with low background absorption and a well-defined spectrum for the extracted theophylline.
P Carter, and
J E Wallace, and
K Blum
September 1975,
Clinical chemistry,
P Carter, and
J E Wallace, and
K Blum
March 1969,
Biochimica et biophysica acta,
P Carter, and
J E Wallace, and
K Blum
July 2013,
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,
P Carter, and
J E Wallace, and
K Blum
February 2014,
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,
P Carter, and
J E Wallace, and
K Blum
February 1978,
Clinical chemistry,
P Carter, and
J E Wallace, and
K Blum
July 1972,
Clinical chemistry,
P Carter, and
J E Wallace, and
K Blum
July 2013,
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,
P Carter, and
J E Wallace, and
K Blum
January 1967,
Scandinavian journal of clinical and laboratory investigation,
P Carter, and
J E Wallace, and
K Blum
January 1963,
The American journal of medical technology,
P Carter, and
J E Wallace, and
K Blum
January 1956,
Scandinavian journal of clinical and laboratory investigation,
