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Isolated frog rod outer segments (ROS) incubated in a Krebs-bicarbonate medium, and illuminated for 2 h, show a profound alteration in their structure. This is characterized by distention of discs, vesiculation, and a marked swelling. The light-induced ROS disruption requires the presence of bicarbonate and sodium chloride. Replacement of bicarbonate by TRIS or HEPES protects ROS structure. Also, substitution of sodium chloride by sucrose or choline chloride maintains unaltered the ROS structure. Deletion of calcium, magnesium, or phosphate does not modify the effect produced by illumination. An increased accumulation of labeled bicarbonate and tritiated water is observed in illuminated ROS, as compared with controls in the dark. The presence of taurine, GABA, or glycine, at concentrations of 5-25 mM, effectively counteracts the light-induced ROS disruption. Taurine (25 mM) reduces labeled bicarbonate and tritiated water levels to those observed in the dark incubated ROS.
H Pasantes-Morales, and
R M Ademe, and
O Quesada
June 1981,
Experimental eye research,
H Pasantes-Morales, and
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October 1977,
Brain research,
H Pasantes-Morales, and
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March 1985,
Neuroscience letters,
H Pasantes-Morales, and
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December 1979,
The Journal of general physiology,
H Pasantes-Morales, and
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November 1982,
Fiziologicheskii zhurnal SSSR imeni I. M. Sechenova,
H Pasantes-Morales, and
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January 2000,
Advances in experimental medicine and biology,
H Pasantes-Morales, and
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January 1982,
Vision research,
H Pasantes-Morales, and
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April 1985,
Biochemical and biophysical research communications,
H Pasantes-Morales, and
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January 1993,
Nippon Ganka Gakkai zasshi,
H Pasantes-Morales, and
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April 1979,
Photochemistry and photobiology,
