BIOMAT Database Logo BIOMAT Database Logo

Molecular cloning of murine endogenous viral sequences and expression of a newly constructed recombinant murine leukemia virus DNA in transfected mink cells. 1982

F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers

In the process of molecularly cloning unintegrated proviral DNA from NIH-3T3 mouse cells infected with Rauscher murine leukemia virus, we observed the occurrence of clones with inserts smaller than the expected Rauscher murine leukemia virus fragments. The insert of one of these clones, lambda.Xe-1, was characterized in more detail. It had a size of 3.5 kilobases. The restriction map was similar but not identical to that of the envelope regions of Moloney and Rauscher murine leukemia viruses. After ligation to previously cloned Moloney murine leukemia viral sequences and transfection of the ligated DNA into mink lung cells a nondefective xenotropic murine leukemia virus, XH-19, was isolated. Restriction mapping of proviral DNA isolated from mink lungs cells chronically infected with XH-19 showed the presence of Moloney murine leukemia virus-derived sequences coupled to xenotropic viral sequences.

UI MeSH Term Description Entries
D008907 Mink Carnivores of genera Mustela and Neovison of the family MUSTELIDAE. The European mink has white upper and lower lips while the American mink lacks white upper lip. American Mink,European Mink,Mustela lutreola,Mustela macrodon,Mustela vison,Neovison vison,Sea Mink,Mink, American,Mink, European,Mink, Sea,Minks,Minks, Sea,Sea Minks,vison, Neovison
D009052 Leukemia Virus, Murine Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice. Graffi Virus,Graffi's Chloroleukemic Strain,Leukemia Viruses, Murine,Mouse Leukemia Viruses,Murine Leukemia Virus,Murine Leukemia Viruses,Graffi Chloroleukemic Strain,Graffis Chloroleukemic Strain,Leukemia Viruses, Mouse
D010582 Bacteriophage lambda A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection. Coliphage lambda,Enterobacteria phage lambda,Phage lambda,lambda Phage
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005814 Genes, Viral The functional hereditary units of VIRUSES. Viral Genes,Gene, Viral,Viral Gene
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Expression of endogenous murine leukemia virus in transplantable tumor cells and DNA methylation of the viral sequences.
August 1984, Cancer research,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Restriction analysis and molecular cloning of endogenous murine leukemia virus-specific DNA sequences of the mouse genome.
November 1982, Virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Unintegrated murine leukemia viral DNA in newly infected cells.
November 1974, Virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Molecular cloning and characterization of murine leukemia virus-related DNA sequences from C3H/HeN mouse DNA.
July 1982, Journal of virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Nucleotide sequence analysis establishes the role of endogenous murine leukemia virus DNA segments in formation of recombinant mink cell focus-forming murine leukemia viruses.
June 1984, Journal of virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Distribution of endogenous murine leukemia virus DNA sequences among mouse chromosomes.
March 1980, Journal of virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Mobility of endogenous ecotropic murine leukemia viral genomes within mouse chromosomal DNA and integration of a mink cell focus-forming virus-type recombinant provirus in the germ line.
March 1982, Journal of virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells.
January 1980, Proceedings of the National Academy of Sciences of the United States of America,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Molecular cloning of B- and N-tropic endogenous BALB/c murine leukemia virus circular DNA intermediates: isolation and characterization of infectious recombinant clones.
July 1981, Journal of virology,
F A van der Hoorn, and C Onnekink, and H van der Putten, and M Zijlstra, and H P Bloemers
Viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells.
September 1978, Journal of virology,
Copied contents to your clipboard!