BIOMAT Database Logo BIOMAT Database Logo

Morphologic observations of contact-induced lysis of EBV-infected B lymphocytes by autologous hand mirror T cells. 1982

W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong

To study the possible immunologic role of hand mirror lymphocytes (HML) in the control of Epstein-Barr virus (EBV)-infected B lymphocytes in patients with infectious mononucleosis (IM), we studied the in vitro interactions of these cells by time-lapse video light microscopy. Peripheral blood T lymphocytes isolated from three patients in the early recovery phase of IM were mixed with their autologous EBV-infected B cells. Motile lymphocytes with their characteristic hand mirror shape were observed to attach by their uropods to the B cells. The HML remained attached for variable periods ranging from 45-75 min. Following detachment, B cells that were in contact with HML underwent lysis. Mixtures of T cells from healthy donors and EBV-infected cell lines exhibited only rare uropod formation with no attachment or lysis of B cells. The present experiment indicates that contact-induced lysis of EBV-infected B lymphocytes is operative in IM and that this process is mediated by the HML.

UI MeSH Term Description Entries
D007244 Infectious Mononucleosis A common, acute infection usually caused by the Epstein-Barr virus (HERPESVIRUS 4, HUMAN). There is an increase in mononuclear white blood cells and other atypical lymphocytes, generalized lymphadenopathy, splenomegaly, and occasionally hepatomegaly with hepatitis. Glandular Fever,Mononucleosis, Infectious,Fever, Glandular
D007694 Killer Cells, Natural Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type. NK Cells,Natural Killer Cells,Cell, NK,Cell, Natural Killer,Cells, NK,Cells, Natural Killer,Killer Cell, Natural,NK Cell,Natural Killer Cell
D002450 Cell Communication Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP. Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D003602 Cytotoxicity, Immunologic The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement. Tumoricidal Activity, Immunologic,Immunologic Cytotoxicity,Immunologic Tumoricidal Activities,Immunologic Tumoricidal Activity,Tumoricidal Activities, Immunologic
D004854 Herpesvirus 4, Human The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies. Burkitt Herpesvirus,Burkitt Lymphoma Virus,E-B Virus,EBV,Epstein-Barr Virus,Human Herpesvirus 4,Infectious Mononucleosis Virus,Burkitt's Lymphoma Virus,HHV-4,Herpesvirus 4 (gamma), Human,Burkitts Lymphoma Virus,E B Virus,E-B Viruses,Epstein Barr Virus,Herpesvirus, Burkitt,Infectious Mononucleosis Viruses,Lymphoma Virus, Burkitt,Mononucleosis Virus, Infectious,Mononucleosis Viruses, Infectious
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Recognition of B-CLL cells experimentally infected with EBV by autologous T lymphocytes.
February 1998, Immunology letters,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Stimulation of human peripheral blood lymphocytes by autologous EBV-infected B cells.
November 1978, Cellular immunology,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Lysis of autologous Epstein-Barr virus-infected B cells by cytotoxic T lymphocytes of rheumatoid arthritis patients.
July 1982, Clinical immunology and immunopathology,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Lysis of infected myofibers by coxsackievirus B-3-immune T lymphocytes.
March 1980, The American journal of pathology,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Morphologic observations of human T and B lymphocytes by scanning electron microscopy.
December 1974, Acta medica Okayama,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
EBV lytic infection enhances transformation of B-lymphocytes infected with EBV in the presence of T-lymphocytes.
March 2012, Journal of medical virology,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Lysis of fresh human tumor cells by autologous large granular lymphocytes and T-lymphocytes: two distinct killing activities induced by coculture with autologous tumor.
December 1984, Journal of the National Cancer Institute,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Major histocompatibility complex-restricted lysis of neuroblastoma cells by autologous cytotoxic T lymphocytes.
January 2001, Journal of immunotherapy (Hagerstown, Md. : 1997),
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Morphological observations on the contact-induced lysis of target cells.
January 1973, European journal of immunology,
W J Thomas, and B Duval-Arnould, and W J Creegan, and H R Schumacher, and D S Forman, and D M Strong
Innate immune control of EBV-infected B cells by invariant natural killer T cells.
October 2013, Blood,
Copied contents to your clipboard!