-transparent.png){kind=logo}
We describe conditions for determining angiotensin converting enzyme (EC 3.4.15.1) in serum, by liquid chromatography. Serum (10 microL) is assayed with the artificial substrate hippuryl-glycyl-glycine (30 mmol/L) in a 50 mmol/L "HEPES" buffer solution with a high salt content (300 mmol of NaCl and 400 mmol of Na2SO4 per liter), at pH 8.0. The resulting enzymic activity is ninefold that of the currently popular spectrophotometric assay of Cushman and Cheung as modified by Lieberman (Am. J. Med. 59: 365-372, 1975). The hippuric acid end product is separated from the substrate by reversed-phase liquid chromatography and measured spectrophotometrically at 228 nm. o-Methyl hippuric acid is used as internal standard. The mean value for 100 normal control subjects was 317 (SD 96) nmol of hippuric acid released per milliliter of serum per minute. The enzyme activity is greater in newborns (p less than 0.05) and has a tendency to decrease with age. This partly automated method, which is optimized with regard to activity and detection, can be used in clinical routine.
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
December 1984,
Clinica chimica acta; international journal of clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
December 1981,
Clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
August 1976,
American journal of clinical pathology,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
June 1983,
Clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
October 1981,
Clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
March 1993,
Clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
August 1984,
Clinical science (London, England : 1979),
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
May 1986,
Clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
April 1979,
Clinica chimica acta; international journal of clinical chemistry,
H M Neels, and
S L Scharpé, and
M E van Sande, and
R M Verkerk, and
K J Van Acker
January 1999,
Thorax,
