-transparent.png){kind=logo}
The solid-phase immune electron microscopy-double-antibody technique, which takes less than 1 h to perform, was applied as a rapid, sensitive, and specific diagnostic tool in the demonstration of papovavirus particles. BK virus propagated in 82C human skin fibroblasts and a monospecific high-titer immune serum to BK virus were used to establish the test procedure. When Formvar-carbon-coated grids were treated with appropriately diluted antibody, a 28-fold increase of virus particles per square micrometer was observed. Viewing of the virus particles was facilitated by the addition of a second "decorator" antibody. BK virus preparations at concentrations of 10(2) to 10(3) PFU/ml could be detected by this technique. There was no cross-reaction with mouse polyomavirus.
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
January 1980,
Journal of immunological methods,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
May 1990,
Journal of clinical microbiology,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
February 1984,
Journal of clinical microbiology,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
February 1982,
Journal of virological methods,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
May 1987,
Journal of virological methods,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
July 1987,
Journal of virological methods,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
May 1988,
Research in veterinary science,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
January 1984,
Archives of virology,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
February 1969,
Laboratory investigation; a journal of technical methods and pathology,
G Giraldo, and
E Beth, and
J Lee, and
E de Harven, and
M Chernesky
July 1981,
The Journal of general virology,
