Biomaterial Database

[Non-specific acid nucleoside triphosphatase from cytosol and chromatin of rat liver: partial purification and general properties]. 1982

S N Naryzhnyĭ, and V M Krutiakov

The nucleoside triphosphatase (EC 3.6.1.15) was isolated from rat liver cytosol and purified 600-fold. The enzyme hydrolyzes all NTPs and dNTPs, splits NDPs and dNDPs at a low rate and does not destroy NMPs and dNMPs. The phosphatase consists of a single subunit with molecular weight of 65 000. The chromatin fraction of the enzyme is fully bound to the membrane, whereas the cytosol fraction contains 15-30% of the membrane-bound enzyme. Both free and membrane-bound phosphatases possess identical functional properties. The enzymatic activity has a pH-optimum of 4.0--4.5, is increased in the presence of Me2+ and is unaffected by ouabain, Triton X-100, N-ethylmaleimide, NaF or DNA, but is inhibited by NaCl, Pi and PPi. The value of Km is equal to 20 microM for TTP splitting. Since the NTP pool is essentially changed throughout the cell cycle, it is suggested that the nucleoside triphosphatase can participate in the nucleotide pool regulation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D010744	Phosphoric Monoester Hydrolases	A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate.	Phosphatase,Phosphatases,Phosphohydrolase,Phosphohydrolases,Phosphomonoesterase,Phosphomonoesterases,Phosphoric Monoester Hydrolase,Hydrolase, Phosphoric Monoester,Hydrolases, Phosphoric Monoester,Monoester Hydrolase, Phosphoric
D002413	Cations, Divalent	Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.	Divalent Cations
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002843	Chromatin	The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.	Chromatins
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D043583	Nucleoside-Triphosphatase	An enzyme which catalyzes the hydrolysis of nucleoside triphosphates to nucleoside diphosphates. It may also catalyze the hydrolysis of nucleotide triphosphates, diphosphates, thiamine diphosphates and FAD. The nucleoside triphosphate phosphohydrolases I and II are subtypes of the enzyme which are found mostly in viruses.	NTPase,Nucleoside Triphosphatase,Nucleoside Triphosphate Phosphohydrolase,Nucleoside Triphosphate Phosphohydrolase I,Nucleoside Triphosphate Phosphohydrolase II,Nucleosidetriphosphatase,Phosphohydrolase, Nucleoside Triphosphate,Triphosphatase, Nucleoside,Triphosphate Phosphohydrolase, Nucleoside