BIOMAT Database Logo BIOMAT Database Logo

Generation of leukotriene B4, its all trans isomers and 5-hydroxyeicosatetraenoic acid by rat basophilic leukaemia cells. 1982

A W Ford-Hutchinson, and P J Piper, and M N Samhoun

1 When rat basophilic leukaemia (RBL-1) cells were incubated for 4 min, in the presence of arachidonic acid (AA) and calcium ionophore A23187, three isomers of leukotriene (LT) B4 and 5-hydroxyeicosatetraenoic acid (5-HETE) were generated. 2 The isomers of LTB4 separated by reverse phase high pressure liquid chromatography (h.p.l.c.) were found to be 5 (s)-dihydroxy-6, 14-trans-8,10-trans-eicosatetraenoic acid: 5(S), 12(R)-dihydroxy-6,14-trans-8,10-trans-eicosatetraenoic acid and 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid or LTB4. 3 5(S), 12(R)-cis, trans, trans-LTB4 produced by RBL-1 cells caused aggregation of rat polymorphonuclear leucocytes (PMNs). Its other two isomers (5(S), 12(S)-all trans-LTB4; 5(S), 12-(R)-all trans-LTB4) were much less active and 5-HETE was found to be inactive. 4 5(S), 12(R)-cis, trans, trans-LTB4 formed from RBL-1 cells contracted guinea-pig parenchymal strips (GPPs) and was indistinguishable from synthetic 5(S), 12(R)-cis, trans, trans-LTB4. The other two isomers of LTB4. i.e. 5(S), 12(S)-all trans-LTB4 and 5(S), 12(R)-all trans-LTB4 as well as 5-HETE were inactive on GPP. 5 RBL-1 LTB4 was equipotent with LTD4. in most experiments, in contracting GPP but had no activity on guinea-pig ileum smooth muscle (GPISM). 6 RBL-1 cells contain the enzyme systems necessary for generating LTB4 as well as the leukotriene(s) with amino acid residues at C-6.

UI MeSH Term Description Entries
D007942 Leukemia, Experimental Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues. Experimental Leukemia,Experimental Leukemias,Leukemia Model, Animal,Leukemias, Experimental,Animal Leukemia Model,Animal Leukemia Models,Leukemia Models, Animal
D007975 Leukotriene B4 The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990) 5,12-HETE,5,12-diHETE,LTB4,Leukotriene B,Leukotriene B-4,Leukotrienes B,5,12 HETE,5,12 diHETE,B-4, Leukotriene,Leukotriene B 4
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D002449 Cell Aggregation The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type. Aggregation, Cell,Aggregations, Cell,Cell Aggregations
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006893 Hydroxyeicosatetraenoic Acids Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes. HETE,Acids, Hydroxyeicosatetraenoic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001095 Arachidonic Acids Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic
D001491 Basophils Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes. Basophil

Related Publications

A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Transformation and degradation of leukotriene B4, 12-hydroxyeicosatetraenoic acid and 5-hydroxyeicosatetraenoic acid induced by ultraviolet radiation.
February 1992, Photodermatology, photoimmunology & photomedicine,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Activation of neutrophil chemotaxis by leukotriene B4 and 5-hydroxyeicosatetraenoic acid in chronic inflammatory bowel disease.
October 1987, Scandinavian journal of clinical and laboratory investigation,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Pasteurella haemolytica leukotoxin enhances production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by bovine polymorphonuclear leukocytes.
August 1992, Infection and immunity,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Enzymatic conversion of leukotriene B4 to 6-trans-leukotriene B4 by rat kidney homogenates.
February 1987, Biochemical and biophysical research communications,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by human neutrophils is inhibited by Pseudomonas aeruginosa phenazine derivatives.
September 1991, Infection and immunity,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
A human epithelial cell line, intestine 407, can produce 5-hydroxyeicosatetraenoic acid and leukotriene B4.
January 1993, Prostaglandins,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Conversion of 5-hydroperoxyeicosatetraenoic acid into leukotriene B4 by rat hepatocytes: a novel cellular source for leukotriene B4.
September 1988, Molecular pharmacology,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
In vivo effects of 1,25-dihydroxyvitamin D3 and minerals on synthesis of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by rat peritoneal neutrophils.
April 1988, Prostaglandins, leukotrienes, and essential fatty acids,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Serum leukotriene B4 and hydroxyeicosatetraenoic acid in the prediction of pre-eclampsia.
January 2021, Placenta,
A W Ford-Hutchinson, and P J Piper, and M N Samhoun
Rat alveolar macrophages synthesize leukotriene B4 and 12-hydroxyeicosatetraenoic acid from alveolar epithelial cell-derived arachidonic acid.
October 1988, The American review of respiratory disease,
Copied contents to your clipboard!