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Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fractions was assessed by electron microscopy and biochemical assays. The membrane subfraction retained 15% of content proteins and lipids, and these could not be removed by various washing procedures. The content subfraction was contaminated by both membrane fragments and vesicles and accounted for 5 to 10% of the membrane enzyme activities of the original Golgi fraction. The lipid compositions of the subfractions was determined, and the phospholipids of both membrane and content were found to be uniformly labeled with [33P]phosphate administered in vivo.
K E Howell, and
G E Palade
March 1978,
Experimental cell research,
K E Howell, and
G E Palade
January 1980,
The Journal of cell biology,
K E Howell, and
G E Palade
September 1984,
Biochemical and biophysical research communications,
K E Howell, and
G E Palade
July 1983,
Biokhimiia (Moscow, Russia),
K E Howell, and
G E Palade
January 1986,
Methods in enzymology,
K E Howell, and
G E Palade
June 1998,
Electrophoresis,
K E Howell, and
G E Palade
January 1982,
The Journal of cell biology,
K E Howell, and
G E Palade
January 1955,
Experimental cell research,
K E Howell, and
G E Palade
February 1996,
Current eye research,
K E Howell, and
G E Palade
January 1984,
Experimental pathology,
