BIOMAT Database Logo BIOMAT Database Logo

Molecular cloning of Rhizobium trifolii genes involved in symbiotic nitrogen fixation. 1982

K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine

DNA sequences responsible for the development and maintenance of symbiotic nitrogen fixation have been identified and isolated from Rhizobium trifolii. Symbiotically-defective strains were generated by random mutagenesis with the transposon Tn5. The defective genes which give rise to the mutant phenotype have been cloned into bacterial plasmids and used as hybridization probes to isolate the corresponding wild-type genes from a clone bank of R. trifolii DNA. Symbiotic genes cloned in this manner are able to correct the lesion caused by the insertion of the transposon in their respective mutants and so restore the nitrogen fixation phenotype. The correction of the mutation is shown to occur by two distinguishable mechanisms--either by complementation or by homologous recombination. This approach provides a reliable method for isolation and mapping of bacterial DNA sequences involved in symbiotic nitrogen fixation.

UI MeSH Term Description Entries
D007887 Fabaceae The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family. Afzelia,Amorpha,Andira,Baptisia,Callerya,Ceratonia,Clathrotropis,Colophospermum,Copaifera,Delonix,Euchresta,Guibourtia,Legumes,Machaerium,Pithecolobium,Stryphnodendron,Leguminosae,Pea Family,Pithecellobium,Tachigalia,Families, Pea,Family, Pea,Legume,Pea Families
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D009586 Nitrogen Fixation The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds. Diazotrophy,Diazotrophic Activity,Dinitrogen Fixation,N2 Fixation,Activities, Diazotrophic,Activity, Diazotrophic,Diazotrophic Activities,Fixation, Dinitrogen,Fixation, N2,Fixation, Nitrogen
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D010946 Plants, Medicinal Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals. Herbs, Medicinal,Medicinal Herbs,Healing Plants,Medicinal Plants,Pharmaceutical Plants,Healing Plant,Herb, Medicinal,Medicinal Herb,Medicinal Plant,Pharmaceutical Plant,Plant, Healing,Plant, Medicinal,Plant, Pharmaceutical,Plants, Healing,Plants, Pharmaceutical
D011995 Recombination, Genetic Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses. Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004251 DNA Transposable Elements Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. DNA Insertion Elements,DNA Transposons,IS Elements,Insertion Sequence Elements,Tn Elements,Transposable Elements,Elements, Insertion Sequence,Sequence Elements, Insertion,DNA Insertion Element,DNA Transposable Element,DNA Transposon,Element, DNA Insertion,Element, DNA Transposable,Element, IS,Element, Insertion Sequence,Element, Tn,Element, Transposable,Elements, DNA Insertion,Elements, DNA Transposable,Elements, IS,Elements, Tn,Elements, Transposable,IS Element,Insertion Element, DNA,Insertion Elements, DNA,Insertion Sequence Element,Sequence Element, Insertion,Tn Element,Transposable Element,Transposable Element, DNA,Transposable Elements, DNA,Transposon, DNA,Transposons, DNA
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA

Related Publications

K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Symbiotic nitrogen fixation: molecular cloning of Rhizobium genes involved in exopolysaccharide synthesis and effective nodulation.
January 1982, Journal of molecular and applied genetics,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Host-specific regulation of symbiotic nitrogen fixation in Rhizobium leguminosarum biovar trifolii.
September 2007, Microbiology (Reading, England),
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Reiteration of genes involved in symbiotic nitrogen fixation by fast-growing Rhizobium japonicum.
November 1984, Journal of bacteriology,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Molecular genetics of Rhizobium Meliloti symbiotic nitrogen fixation.
January 1989, Biotechnology advances,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Respiratory Enzyme Systems in Symbiotic Nitrogen Fixation: III. The Dehydrogenase Systems of Rhizobium trifolii and Rhizobium leguminosarum.
April 1941, Journal of bacteriology,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Characterization of the cycHJKL genes involved in cytochrome c biogenesis and symbiotic nitrogen fixation in Rhizobium leguminosarum.
September 1995, Journal of bacteriology,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Rhizobium japonicum mutants defective in symbiotic nitrogen fixation.
October 1982, Journal of bacteriology,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical.
April 1993, Journal of bacteriology,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
[symbiotic fixation of molecular nitrogen].
January 1974, Izvestiia Akademii nauk SSSR. Seriia biologicheskaia,
K F Scott, and J E Hughes, and P M Gresshoff, and J E Beringer, and B G Rolfe, and J Shine
Control of the expression of bacterial genes involved in symbiotic nitrogen fixation.
July 1993, World journal of microbiology & biotechnology,
Copied contents to your clipboard!