BIOMAT Database Logo BIOMAT Database Logo

The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. 1982

Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov

The primary structure of the E. coli rpoC gene (5321 base pairs) coding the beta'-subunit of RNA polymerase as well as its adjacent segment have been determined. The structure analysis of the peptides obtained by cleavage of the protein with cyanogen bromide and trypsin has confirmed the amino acid sequence of the beta'-subunit deduced from the nucleotide sequence analysis. The beta'-subunit of E. coli RNA polymerase contains 1407 amino acid residues. Its translation is initiated by codon GUG and terminated by codon TAA. It has been detected that the sequence following the terminating codon is strikingly homologous to known sequences of rho-independent terminators.

UI MeSH Term Description Entries
D009876 Operon In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION. Operons
D003062 Codon A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE). Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D014176 Protein Biosynthesis The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS. Genetic Translation,Peptide Biosynthesis, Ribosomal,Protein Translation,Translation, Genetic,Protein Biosynthesis, Ribosomal,Protein Synthesis, Ribosomal,Ribosomal Peptide Biosynthesis,mRNA Translation,Biosynthesis, Protein,Biosynthesis, Ribosomal Peptide,Biosynthesis, Ribosomal Protein,Genetic Translations,Ribosomal Protein Biosynthesis,Ribosomal Protein Synthesis,Synthesis, Ribosomal Protein,Translation, Protein,Translation, mRNA,mRNA Translations
D046911 Macromolecular Substances Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure. Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular

Related Publications

Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Primary structure of Escherichia coli RNA-polymerase. The nucleotide sequence of the rpoC gene and the amino acid sequence of the beta'-subunit].
January 1981, Doklady Akademii nauk SSSR,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Primary structure of Escherichia coli RNA-polymerase. Nucleotide sequence of gene rpoB and amino acid sequence of the beta-subunit].
January 1980, Doklady Akademii nauk SSSR,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC).
December 1981, Nucleic acids research,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Nucleotide sequence of the rpoC-gene coding for RNA polymerase beta'-subunit of Pseudomonas putida].
January 1988, Doklady Akademii nauk SSSR,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
Nucleotide sequence of the proximal portion of the RNA polymerase beta subunit gene of Escherichia coli.
November 1980, Gene,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Primary structure of the E. coli DNA-dependent RNA-polymerase beta'-subunit. Hydrolysis with trypsin].
June 1984, Bioorganicheskaia khimiia,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.
June 1981, Nucleic acids research,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
April 1979, Proceedings of the National Academy of Sciences of the United States of America,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Nucleotide sequence of the Salmonella typhimurium rpoB gene encoding the beta-subunit of RNA polymerase].
January 1986, Doklady Akademii nauk SSSR,
Ovchinnikov YuA, and G S Monastyrskaya, and V V Gubanov, and S O Guryev, and I S Salomatina, and T M Shuvaeva, and V M Lipkin, and E D Sverdlov
[Nucleotide sequence of of rpoB gene encoding the RNA-polymerase beta-subunit in Pseudomonas putida].
January 1988, Doklady Akademii nauk SSSR,
Copied contents to your clipboard!