-transparent.png){kind=logo}
We have examined the chromatin structure of the centromere regions of chromosomes III and XI in yeast by using cloned functional centromere DNAs (CEN3 and CEN11) as labeled probes. When chromatin from isolated nuclei is digested with micrococcal nuclease and the resulting DNA fragments separated electrophoretically and blotted to nitrocellulose filters, the centromeric nucleosomal subunits are resolved into significantly more distinct ladders than are those from the bulk of the chromatin. A discrete protected region of 220-250 bp of CEN sequence flanked by highly nuclease-sensitive sites was revealed by mapping the exact nuclease cleavage sites within the centromeric chromatin. On both sides of this protected region, highly phased and specific nuclease cutting sites exist at nucleosomal intervals (160 bp) for a total length of 12-15 nucleosomal subunits. The central protected region in the chromatin of both centromeres spans the 130 bp segment that exhibits the highest degree of sequence homology (71%) between functional CEN3 and CEN11 DNAs. This unique chromatin structure is maintained on CEN sequences introduced into yeast on autonomously replicating plasmids, but is not propagated through foreign DNA sequences flanking the inserted yeast DNA.
K S Bloom, and
J Carbon
October 1980,
Nature,
K S Bloom, and
J Carbon
January 2001,
The Journal of biological chemistry,
K S Bloom, and
J Carbon
August 1993,
Molecular biology of the cell,
K S Bloom, and
J Carbon
November 1981,
Gene,
K S Bloom, and
J Carbon
August 1986,
Proceedings of the National Academy of Sciences of the United States of America,
K S Bloom, and
J Carbon
August 1991,
Cell,
K S Bloom, and
J Carbon
January 2012,
PloS one,
K S Bloom, and
J Carbon
April 1995,
Gene,
K S Bloom, and
J Carbon
June 1989,
Current genetics,
K S Bloom, and
J Carbon
December 2016,
Virology,
