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We succeeded in isolating human rotaviruses from the feces of gastroenteritis patients by using roller cultures of primary cynomolgus monkey kidney cells with trypsin in the maintenance medium but without concentration and trypsin treatment of the inocula at each passage level. These cells were found to be more sensitive than MA-104 cells (derived from fetal rhesus monkey kidney) for the propagation of human rotaviruses. Polyacrylamide gel electrophoresis of the genome RNA revealed that there were small differences in the migration pattern of the segments among all the strains isolated from 1976 to 1981. The cultivation of human rotaviruses in primary cell cultures might aid in developing a liver rotavirus vaccine.
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
November 1986,
Zhonghua yi xue za zhi,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
April 1983,
Veterinary microbiology,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
September 1989,
Journal of virological methods,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
February 1994,
Acta virologica,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
June 1955,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
September 1982,
Acta virologica,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
January 1973,
Acta microbiologica Polonica. Series A: Microbiologia generalis,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
June 1955,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
December 1965,
The Journal of infectious diseases,
A Hasegawa, and
S Matsuno, and
S Inouye, and
R Kono, and
Y Tsurukubo, and
A Mukoyama, and
Y Saito
January 1986,
Avian diseases,
