Biomaterial Database

[Circular dichroism study of the ribonucleoprotein structure of Sendai virus isolated from infected chick embryo cells]. 1982

T L Busse, and N G Iaroslavtseva, and M L Khristova

Thermostability and secondary structure of RNA, a component of ribonucleoprotein (RNP) of Sendai virus isolated from infected chick embryo cells (cRNP) and from virions (vRNP) were studied by the method of circular dichroism. The secondary structure of RNA in cRNP was shown to be practically no different from that of free RNA whereas incorporation of RNA into vRNP was accompanied by a significant decrease in the number of paired bases in it. Comparison of thermodenaturation parameters of cRNP and vRNP also revealed significant differences in their structural organization. Thus, melting of cRNP as well as of free RNA is of markedly non-cooperative nature indicating poor RNA-protein interactions in the complex. In contrast, the process of vRNP thermodenaturation occurs in a step-wise manner in a narrow temperature range indicating a significant role in the maintenance of this RNP structure of both RNA-protein and, apparently, protein-protein interactions.

UI	MeSH Term	Description	Entries
D009698	Nucleoproteins	Proteins conjugated with nucleic acids.	Nucleoprotein
D010222	Parainfluenza Virus 1, Human	A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.	Hemadsorption Virus 2,Human parainfluenza virus 1,Para-Influenza Virus Type 1,Parainfluenza Virus Type 1,Para Influenza Virus Type 1
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D002942	Circular Dichroism	A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Circular Dichroism, Vibrational,Dichroism, Circular,Vibrational Circular Dichroism
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012261	Ribonucleoproteins	Complexes of RNA-binding proteins with ribonucleic acids (RNA).	Ribonucleoprotein
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D014771	Virion	The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.	Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D018184	Paramyxoviridae Infections	Infections with viruses of the family PARAMYXOVIRIDAE. This includes MORBILLIVIRUS INFECTIONS; RESPIROVIRUS INFECTIONS; PNEUMOVIRUS INFECTIONS; HENIPAVIRUS INFECTIONS; AVULAVIRUS INFECTIONS; and RUBULAVIRUS INFECTIONS.	Parainfluenza,Parainfluenza Virus Infections,Infections, Paramyxoviridae,Infection, Parainfluenza Virus,Infection, Paramyxoviridae,Infections, Parainfluenza Virus,Parainfluenza Virus Infection,Paramyxoviridae Infection,Virus Infection, Parainfluenza,Virus Infections, Parainfluenza