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A radioimmunoassay for leukotriene B4 has been developed. The assay is sensitive; 5 pg LTB4 caused significant inhibition of binding of [3H]-LTB4 and 50% displacement occurred with 30 pg. The specificity of the assay has been critically examined; prostaglandins, thromboxane B2 and arachidonic acid do not exhibit detectable cross-reactions (less than 0.03%). However, some non-cyclic dihydroxy- and monohydroxy-eicosatetraenoic acids do cross-react slightly (e.g. diastereomers of 5,12-dihydroxy-6,8,10-trans-14-cis-eicosatetraenoic and 12-hydroxy-5,8,10,14-eicosatetraenoic acids cross-react 3.3% and 2.0% respectively). The assay has been used to monitor the release of LTB4 from human neutrophils in response to the divalent cation ionophore, A23187. The immunoreactive material released during these incubations was confirmed as LTB4 by reverse phase high pressure liquid chromatography following solvent extraction and silicic acid chromatography.
J A Salmon, and
P M Simmons, and
R M Palmer
December 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J A Salmon, and
P M Simmons, and
R M Palmer
October 1983,
Prostaglandins,
J A Salmon, and
P M Simmons, and
R M Palmer
January 1985,
Advances in prostaglandin, thromboxane, and leukotriene research,
J A Salmon, and
P M Simmons, and
R M Palmer
February 1998,
The international journal of biochemistry & cell biology,
J A Salmon, and
P M Simmons, and
R M Palmer
October 1990,
Sheng li ke xue jin zhan [Progress in physiology],
J A Salmon, and
P M Simmons, and
R M Palmer
August 2002,
Prostaglandins & other lipid mediators,
J A Salmon, and
P M Simmons, and
R M Palmer
January 1987,
Progress in clinical and biological research,
J A Salmon, and
P M Simmons, and
R M Palmer
April 1988,
FEBS letters,
J A Salmon, and
P M Simmons, and
R M Palmer
June 1982,
Biochemical and biophysical research communications,
