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The equivalent of 5-7 units of platelets, isolated from a single donor with the IBM Blood Cell Processor 2997 using a dual stage separation chamber, was frozen with the cryoprotectant dimethylsulfoxide (DMSO). The DMSO-saline solution was added directly to the platelets, and the platelets were frozen in a polyvinyl chloride plastic bag by storage in a -80 degrees C mechanical freezer. Washing the thawed platelets with a phosphate-buffered sodium chloride-dextrose solution, pH of 5.0, removed about 95% of the DMSO. In vitro freeze-thaw-wash recovery was 80%, and in vivo 51Cr platelet recovery was 31%. Platelet dense body granules were well maintained after freezing, thawing, and washing. This is a safe and effective method of platelet cryopreservation which can be performed in less time than other currently used methods.
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
January 1985,
Journal of clinical apheresis,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
February 1983,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
July 1983,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
January 1983,
Journal of clinical apheresis,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
July 1986,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
January 1986,
Folia haematologica (Leipzig, Germany : 1928),
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
January 1984,
Journal of clinical apheresis,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
August 1981,
American journal of clinical pathology,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
January 1981,
Transfusion,
A J Melaragno, and
W A Abdu, and
R J Katchis, and
J J Vecchione, and
C R Valeri
October 1981,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
