Ribonuclease H activity in cultured plant cells. 1978

Y Sawai, and N Sugano, and K Tsukada

Ribonuclease H (RNAase H) was extracted from cultured plant cells, strain GD-2 and characterized. RNAase H activity in logarithmical growing cells is much higher than that of stationary cells, and the response of RNAase H activity was very similar to that of DNA polymerase after culture. The activities of RNAase, DNAase, phosphodiesterase and alkaline phosphatase decrease parallel with the increase in growth, and increase to stationary phase, contrasting with those of DNA polymerase and RNAase H.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010944 Plants Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE. Plant
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D012260 Ribonucleases Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-. Nucleases, RNA,RNase,Acid Ribonuclease,Alkaline Ribonuclease,Ribonuclease,RNA Nucleases,Ribonuclease, Acid,Ribonuclease, Alkaline

Related Publications

Y Sawai, and N Sugano, and K Tsukada
March 1990, Experientia,
Y Sawai, and N Sugano, and K Tsukada
November 1977, Life sciences,
Y Sawai, and N Sugano, and K Tsukada
January 2001, Methods in molecular biology (Clifton, N.J.),
Y Sawai, and N Sugano, and K Tsukada
March 1974, Plant physiology,
Y Sawai, and N Sugano, and K Tsukada
December 1974, Lloydia,
Y Sawai, and N Sugano, and K Tsukada
January 1978, Ukrainskii biokhimicheskii zhurnal (1978),
Y Sawai, and N Sugano, and K Tsukada
November 1978, Biochemical and biophysical research communications,
Y Sawai, and N Sugano, and K Tsukada
March 2009, The FEBS journal,
Y Sawai, and N Sugano, and K Tsukada
April 1986, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Copied contents to your clipboard!