BIOMAT Database Logo BIOMAT Database Logo

Purification and properties of an acid protease from human ascitic fluid. 1978

H Esumi, and S Sato, and T Sugimura, and N Okasaki

An acid protease was isolated from the ascitic fluid of a patient with ovarian cancer. It was purified about 400-fold to homogeneity by ammonium sulfate fractionation, gel filtration on Sephadex G-200 and DEAE-cellulose column chromatography. Its molecular weight was calculated to be 28 000 by gel filtration, and its isoelectric points was found to be pH 4.1. It showed similar activities on acid-denatured bovine serum albumin and on acid-denatured bovine hemoglobin, and its optimal pH for both substrates was 3.0. Sulfhydryl compounds and metal ions had no apparent effects on this enzyme, but pepstatin was strongly inhibitory.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D010447 Peptide Hydrolases Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES. Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001202 Ascitic Fluid The serous fluid of ASCITES, the accumulation of fluids in the PERITONEAL CAVITY. Peritoneal Effusion,Peritoneal Fluid,Ascitic Fluids,Effusion, Peritoneal,Fluid, Ascitic,Fluid, Peritoneal,Peritoneal Effusions,Peritoneal Fluids

Related Publications

H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification and characterization of alpha 1-acid glycoprotein from ascitic fluid.
February 1970, Analytical biochemistry,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification and properties of an esterase from human breast cyst fluid.
February 1991, Cancer research,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
The purification of mouse monoclonal antibodies from ascitic fluid.
July 1986, Journal of immunological methods,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
[Ascitic fluid from vaccinated rats and its properties].
June 1970, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification and properties of Mucor pusillus acid protease.
April 1968, Journal of bacteriology,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification and properties of an extracellular protease from Myxococcus virescens.
January 1978, Journal of bacteriology,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification and properties of an acid lipase from human gastric juice.
September 1982, Biochimica et biophysica acta,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Phospholipase A2 in human ascitic fluid. Purification, characterization and immunochemical detection.
August 1991, The Biochemical journal,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification of granulocyte neutral protease from human blood and rheumatoid synovial fluid.
August 1975, Biochimica et biophysica acta,
H Esumi, and S Sato, and T Sugimura, and N Okasaki
Purification of monoclonal antibodies from ascitic fluid using preparative electrophoresis.
December 1998, Journal of chromatography. A,
Copied contents to your clipboard!