Biomaterial Database

Phospholipid synthesis in the squid giant axon: enzymes of phosphatidylinositol metabolism. 1983

R M Gould, and W D Spivack, and D Robertson, and M J Poznansky

We examined the properties of several enzymes of phospholipid metabolism in axoplasm extruded from squid giant axons. The following synthetic enzymes, CDP-diglyceride: inositol transferase (EC 2.7.8.11), ATP:diglyceride phosphotransferase, diglyceride kinase (EC 2.7.2.-), and phosphatidylinositol kinase (EC 2.7.1.67), were all present in axoplasm. Phospholipid exchange proteins, which catalyzed the transfer of phosphatidylinositol and phosphatidylcholine between membrane preparations and unilamellar lipid vesicles, were also found. However, we did not find conditions under which the synthesis of CDP-diglyceride, phosphatidylserine, and phosphatidylinositol-4,5-diphosphate could be measured. Subcellular fractionation by differential centrifugation showed that the axoplasmic inositol transferase and phosphatidylinositol kinase activities were largely "microsomal," while the diglyceride kinase and exchange protein activities were primarily "cytosolic."

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010716	Phosphatidylinositols	Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.	Inositide Phospholipid,Inositol Phosphoglyceride,Inositol Phosphoglycerides,Inositol Phospholipid,Phosphoinositide,Phosphoinositides,PtdIns,Inositide Phospholipids,Inositol Phospholipids,Phosphatidyl Inositol,Phosphatidylinositol,Inositol, Phosphatidyl,Phosphoglyceride, Inositol,Phosphoglycerides, Inositol,Phospholipid, Inositide,Phospholipid, Inositol,Phospholipids, Inositide,Phospholipids, Inositol
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D010770	Phosphotransferases	A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.	Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon
D013347	Subcellular Fractions	Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)	Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D049832	Decapodiformes	A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.	Cuttlefish,Illex,Sepiidae,Squid,Todarodes,Cuttlefishs,Decapodiforme,Illices,Squids,Todarode
D051607	CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase	An enzyme that catalyzes the formation of PHOSPHATIDYLINOSITOL and CMP from CDP-DIACYLGLYCEROL and MYOINOSITOL.	CDP-1,2-Diacyl-sn-Glycerol-Myoinositol 3-Phosphatidyltransferase,CDP-DG Inositol Transferase,CDP-Diacylglycerol-Myo-Inositol 3-Phosphatidyltransferase,CDP-Diglyceride-Inositol Transferase,CDP-Diglyceride-Inositoltransferase,CDPdiacylglycerol-Inositol 3-Phosphatidyltransferase,CDPdiglyceride-Inositol Phosphatidyltransferase,Cytidine 5'-Diphosphate 1,2-Diacyl-sn-Glycerol - Inositol Transferase,Inositol Phosphatidyltransferase,Phosphatidylinositol Synthase,3-Phosphatidyltransferase, CDP-1,2-Diacyl-sn-Glycerol-Myoinositol,3-Phosphatidyltransferase, CDP-Diacylglycerol-Inositol,3-Phosphatidyltransferase, CDP-Diacylglycerol-Myo-Inositol,3-Phosphatidyltransferase, CDPdiacylglycerol-Inositol,CDP 1,2 Diacyl sn Glycerol Myoinositol 3 Phosphatidyltransferase,CDP DG Inositol Transferase,CDP Diacylglycerol Inositol 3 Phosphatidyltransferase,CDP Diacylglycerol Myo Inositol 3 Phosphatidyltransferase,CDP Diglyceride Inositol Transferase,CDP Diglyceride Inositoltransferase,CDPdiacylglycerol Inositol 3 Phosphatidyltransferase,CDPdiglyceride Inositol Phosphatidyltransferase,Cytidine 5' Diphosphate 1,2 Diacyl sn Glycerol Inositol Transferase,Phosphatidyltransferase, CDPdiglyceride-Inositol,Phosphatidyltransferase, Inositol
D017853	Phosphotransferases (Alcohol Group Acceptor)	A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.