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Some kinetic data on the inactivation of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) by betapropiolactone (BPL) and ultraviolet (UV) irradiation are reported. 0.25% BPL at 37 degrees C for 1 h reduced the titre of EHV1 by greater than 10(3 . 4) and of ERhV1 by greater than 10(4 . 1) TCID50/ml. UV irradiation (334 microW/cm2) produced similar reductions in titre after 2 min. These data were used as a basis for inactivating EHV1 and ERhV1 by the combined action of BPL and UV irradiation. Viruses were exposed to 0.1% BPL for 1 h at 4 degrees C with constant stirring, followed by UV irradiation for 2 min, followed by incubation for 3 h at 37 degrees C. Inactivated EHV1 elicited secondary immune responses only in horses whereas ERhV1 produced primary immune responses in mice (including athymic nu/nu mice), rabbits and probably in horses.
T M Campbell, and
M J Studdert, and
M H Blackney
October 1980,
The Cornell veterinarian,
T M Campbell, and
M J Studdert, and
M H Blackney
June 1995,
The Veterinary record,
T M Campbell, and
M J Studdert, and
M H Blackney
April 2016,
Veterinary journal (London, England : 1997),
T M Campbell, and
M J Studdert, and
M H Blackney
April 2000,
The veterinary quarterly,
T M Campbell, and
M J Studdert, and
M H Blackney
December 2005,
Archives of virology,
T M Campbell, and
M J Studdert, and
M H Blackney
January 2000,
Veterinary microbiology,
T M Campbell, and
M J Studdert, and
M H Blackney
December 1980,
Thrombosis and haemostasis,
T M Campbell, and
M J Studdert, and
M H Blackney
September 1977,
Australian veterinary journal,
T M Campbell, and
M J Studdert, and
M H Blackney
January 2019,
Equine veterinary journal,
T M Campbell, and
M J Studdert, and
M H Blackney
November 2010,
Journal of virology,
