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No cAMP-dependent protein kinase activity is found upon DEAE-cellulose chromatography of mouse fat extracts at the low salt concentration characteristic of the Type I isozyme. The RI detected in fat extracts by photoincorporation of the analog, 8-N3 [32P]cAMP, elutes within the high salt Type II isozyme peak. The multiple charge variants of this photolabeled RI which can be resolved by two-dimensional gel electrophoresis are similar to those of the histoptypically-related cultured cells, SV3T3 and 3T6, which do contain Type I kinase isozyme activity peaks. This high salt-eluting RI may be part of a Type I holoenzyme whose elution properties are altered by interactions with other substances present in the extract.
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
January 1980,
Journal of cyclic nucleotide research,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
November 1984,
Journal of chromatography,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
January 1988,
Methods in enzymology,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
January 1981,
Methods in enzymology,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
December 1979,
The Journal of biological chemistry,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
December 1996,
Biochemistry and molecular biology international,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
April 1989,
FEBS letters,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
June 1997,
Biochemical and biophysical research communications,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
August 2002,
Trends in cardiovascular medicine,
A M Malkinson, and
D S Beer, and
J M Wehner, and
J R Sheppard
March 1991,
Biochemistry,
