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A phi 29 DNA fragment containing gene 3, coding for the 5'-terminal protein, and several other early genes has been cloned in a pBR322 derivative plasmid (pKC30) under the control of the pL promoter of bacteriophage lambda. Four polypeptides of Mr 27000, 18500, 17500 and 12500 were labelled with [35S]methionine after heat induction, accounting for about 15% of the de novo synthesized protein. The Mr 27000 and 12500 proteins were characterized as p3, the 5'-terminal protein, and p4, involved in the control of late transcription, respectively. Protein p3 synthesized in Escherichia coli was active in the in vitro formation of the initiation complex p3-dAMP when supplemented with extracts from Bacillus subtilis infected with a sus3 mutant.
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R Pastrana, and
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M Salas
October 1984,
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Nucleic acids research,
J A García, and
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November 1991,
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J A García, and
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M Salas
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July 1988,
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J A García, and
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J A García, and
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Virology,
J A García, and
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January 1991,
Research in microbiology,
J A García, and
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February 2002,
The Journal of biological chemistry,
J A García, and
R Pastrana, and
I Prieto, and
M Salas
December 1983,
Nucleic acids research,
