-transparent.png){kind=logo}
A 809 bp Sau 3A - Hpa I fragment containing a complete HBsAg gene and fragments 744 bp Hinc II - Hpa I and 712 bp Xba I - Hpa I containing a truncated HBsAg gene lacking the sequence encoding the NH2-terminal hydrophobic domain were prepared from a composite plasmid pHBV933 containing the 3.2 kb Eco RI DNA fragment of the entire HBV/adw genome and inserted into an expression vector pTRP801 to give plasmids pTRP SS-6, pTRP SS-39, and pTRP SS-50, respectively. The growth of a recombinant having pTRP SS-6 was greatly inhibited and the transformant expressed a low level of HBsAg, which is reactive to human anti-HBsAg antibody. Interestingly, the growth of transformants harbouring pTRP SS-39 and pTRP SS-50 was not inhibited and these transformants expressed a considerable level of the HBsAg. Minicells harbouring pTRP SS-6, pTRP SS-39, and pTRP SS-50 formed specific polypeptides of about 24 K, 23 K, and 22 K daltons, respectively.
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
March 1986,
Biochemical and biophysical research communications,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
October 1984,
Gene,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
June 1979,
The Journal of infectious diseases,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
January 1983,
Proceedings of the National Academy of Sciences of the United States of America,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
December 2023,
European review for medical and pharmacological sciences,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
March 1980,
The Journal of infectious diseases,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
November 2014,
The American journal of gastroenterology,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
August 2011,
Gastroenterology,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
June 1985,
Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences,
Y Fujisawa, and
Y Ito, and
R Sasada, and
Y Ono, and
K Igarashi, and
R Marumoto, and
M Kikuchi, and
Y Sugino
October 2005,
Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology,
