Biomaterial Database

A technical note on the histochemical demonstration of G6Pase activity. 1983

I P Maly, and D Sasse

Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.

UI	MeSH Term	Description	Entries
D007854	Lead	A soft, grayish metal with poisonous salts; atomic number 82, atomic weight 207.2, symbol Pb.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008298	Maleates	Derivatives of maleic acid (the structural formula (COO-)-C
D009566	Nitrates	Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.	Nitrate
D005260	Female		Females
D005952	Glucose-6-Phosphatase	An enzyme that catalyzes the conversion of D-glucose 6-phosphate and water to D-glucose and orthophosphate. EC 3.1.3.9.	Glucosephosphatase,Glucose 6-Phosphatase,Glucose-6-Phosphate Phosphohydrolase,Glucose 6 Phosphatase
D005958	Glucosephosphates
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014325	Tromethamine	An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)	Tris Buffer,Trisamine,Trometamol,Tri(hydroxymethyl)aminomethane,Tris(hydroxymethyl)aminomethane,Tris-Magnesium(II)-Potassium Chloride Buffer,Tris-Mg(II)-KCl Buffer,Trizma