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To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
January 1990,
Methods in molecular biology (Clifton, N.J.),
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
January 2017,
Methods in molecular biology (Clifton, N.J.),
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
January 1982,
Zeitschrift fur Parasitenkunde (Berlin, Germany),
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
March 1974,
Cancer,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
January 1992,
Journal of medical primatology,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
November 2011,
Journal of visualized experiments : JoVE,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
September 1983,
Poultry science,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
August 1973,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
March 1972,
Journal of the National Cancer Institute,
M von Knebel Doeberitz, and
G W Bornkamm, and
H zur Hausen
August 1986,
Human genetics,
