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A sensitive, solid phase enzyme immunoassay for the determination of aldosterone in plasma and saliva has been developed. For this purpose anti-aldosterone coated tubes were used. The enzyme marker was a covalently linked aldosterone/horseradish peroxidase conjugate. The assay had a limit of detection of 5 pg/assay tube. The test requires neither centrifuge nor gamma or beta counter, but only a simple photometer. The free/bound separation is based on the solid phase technique with a simple washing step. The normal values for aldosterone in plasma and saliva before and after ACTH application were determined. Saliva, being easily collected by a non-invasive, stress-free technique, is an alternative to plasma. The aldosterone levels in patients with Conn's syndrome were significantly increased in plasma and also in saliva.
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B Neef
August 1979,
Clinical chemistry,
W Hubl, and
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F Hofmann, and
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C D Garten, and
P K Schmidt, and
H J Thiele, and
B Neef
January 1983,
Journal of clinical microbiology,
W Hubl, and
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B Neef
March 1980,
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B Neef
October 1979,
The Journal of endocrinology,
