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Two types for antibodies have been raised against the beta-adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti-idiotypic antibodies. Our data illustrate the interactions of the beta-adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of beta-receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.
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