In order to gain some impressions about the degree to which individual neurons of the pars reticulata of the substantia nigra send long collateral branches to more than one of its three major targets (thalamus, superior colliculus, reticular formation), two, or all three targets were injected with fluorescent dyes (Evan's blue, granular blue, nuclear yellow, propidium iodide) in six squirrel monkeys and four cats. The best results were obtained in the monkey brain with injections of Evan's blue in the thalamus, granular blue in the colliculus and nuclear yellow in the reticular formation. Whereas nigrothalamic and nigroreticular neurons are numerous and widely scattered throughout all parts of the pars reticulata, cells projecting only to the superior colliculus are fewer in number and restricted to a rostral-lateral zone. These results are consistent with earlier data obtained with the horseradish peroxidase method. Although double-labeled cells with projections to both the thalamus and reticular formation occur throughout the pars reticulata, such cells are somewhat more abundant at caudal levels of the nucleus. Cells containing dyes from both the superior colliculus and reticular formation are less common and restricted to the lateral part of the pars reticulata. A small number of cells near the rostral pole of the pars reticulata contain dye from both the tectal and thalamic injection. Typically, less than two dozen cells in any case can be confidently identified as containing all three dyes and these cells are located in the rostrolateral half of the pars reticulata. Fewer than 20% of the labeled nigral cells contain more than one dye. In the cat, thalamic injection of granular blue and tectal injection of nuclear yellow indicate that most nigrotectal cells are located in the middle of the mediolateral expanse of the pars reticulata in its rostral half. Nigrothalamic cells flank the nigrotectal group medially, laterally and caudally. Where these groups border one another, several cells contain both dyes indicating that they project to both the thalamus and colliculus. In both the cats and monkeys, a less extensive cell-labeling occurs in the contralateral nigra with a pattern similar to that in the ipsilateral substantia nigra. The results indicate that several neurons of the substantia nigra's pars reticulata send long collateral branches to two or even all three of the major targets. Many reticulata cells, however, appear to project either to the thalamus, or to the superior colliculus or to the reticular formation.