BIOMAT Database Logo BIOMAT Database Logo

A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus. 1983

E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley

We have constructed a frameshift mutation in the simian virus 40 early region using a novel method of oligonucleotide-directed mutagenesis. The mutated DNA specifies an 84,000-dalton large tumor antigen that consists of approximately equal to 75,000 daltons encoded by the wild-type reading frame and 9,000 daltons, by the alternative reading frame (wild-type large tumor antigen is approximately equal to 82,000 daltons). The frameshifted carboxyl terminus of the protein bears a strong similarity to the same region of polyoma virus middle-sized tumor antigen. We have found that the mutant DNA is unable to replicate when introduced into permissive monkey cells and incapable of transforming nonpermissive mouse cells.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002472 Cell Transformation, Viral An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus. Transformation, Viral Cell,Viral Cell Transformation,Cell Transformations, Viral,Transformations, Viral Cell,Viral Cell Transformations
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein

Related Publications

E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
p300 family members associate with the carboxyl terminus of simian virus 40 large tumor antigen.
January 1997, Journal of virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host range.
May 1985, Journal of virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Genetic and biochemical analysis of transformation-competent, replication-defective simian virus 40 large T antigen mutants.
January 1985, Journal of virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
The simian virus 40 large tumor antigen.
October 1987, Biochimica et biophysica acta,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Correction of a carboxyl terminal simian immunodeficiency virus Nef frameshift mutation restores virus replication in macaques.
June 2010, Virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.
May 1986, Journal of virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.
June 1984, Molecular and cellular biology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
November 1980, Proceedings of the National Academy of Sciences of the United States of America,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Subcellular Localization of simian virus 40 large tumor antigen.
May 1979, Journal of virology,
E D Lewis, and S Chen, and A Kumar, and G Blanck, and R E Pollack, and J L Manley
Properties of simian virus 40 mutants lacking the Asp4-Glu-Asp stretch at the carboxyl-terminus of large T antigen.
October 1987, Virology,
Copied contents to your clipboard!