Biomaterial Database

Identification of a new protein present in vesicular stomatitis virus-infected Chinese hamster ovary cells as a degradation product of viral M protein. 1983

C A Rosen, and P S Cohen, and H L Ennis

In addition to the five previously described vesicular stomatitis virus (VSV) proteins (L, G, N, NS, and M), a protein (Mr 17,500) accumulated late in infection of Chinese hamster ovary cells. The protein, designated M', was a cleavage product of the viral M protein (Mr 26,000) both in vivo and in vitro. (a) M' was precipitated by anti VSV serum, indicating that it is of viral origin. (b) M' peptides generated using Staphylococcus V8 protease or chymotrypsin were shared by M, but not by the other VSV proteins. (c) The conversion of M to M' was enzymatic. The enzyme denoted M protease was heat labile, was inhibited by the serine protease inhibitor phenylmethylsulfonyl fluoride, and its accumulation, which commenced between 2 and 3 hr after infection, required protein synthesis. (d) The amounts of L, G, N, and NS increased in CHO-infected cells while the amount of M increased for only 3-4 hr and decreased thereafter. Since M has been implicated in the inhibition of VSV transcription, it is possible that regulation of the amount of M by degradation is important in the regulation of VSV transcription.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010053	Ovary	The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.	Ovaries
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005260	Female		Females
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014721	Vesicular stomatitis Indiana virus	The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.	Vesicular stomatitis-Indiana virus