Biomaterial Database

Kinetics of phagocytosis of Chlamydia psittaci by mouse fibroblasts (L cells): separation of the attachment and ingestion stages. 1978

G I Byrne

The kinetics of phagocytosis of Chlamydia psittaci (6BC) by monolayers of mouse fibroblasts (L cells) was studied with an assay that distinguished between the attachment and ingestion phases of phagocytosis. At multiplicities of 10 and 100 50% infectious doses (ID50) per L cell, virtually all of the inoculated C. psittaci had been attached and ingested after 60 min at 37 degrees C. At multiplicities of 500 to 5,000 ID50 per L cell, the initial rates of attachment and ingestion of C. psittaci to L cells increased with the multiplicity of infection, but phagocytosis stopped even though many chlamydial cells remained free in suspension and readily available for attachment to the host-cell monolayers. Phagocytosis probably ceased because the L cells were injured when they took up large numbers of chlamydial cells. This injury prevented direct determination of the number of potential binding sites for C. psittaci on each L cell. However, this number is large enough to make the rates of chlamydial attachment and ingestion predominantly dependent on the multiplicity of infection.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007739	L Cells	A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.	Earle's Strain L Cells,L Cell Line,L Cells (Cell Line),L-Cell Line,L-Cells,L-Cells, Cell Line,L929 Cell Line,L929 Cells,NCTC Clone 929 Cells,NCTC Clone 929 of Strain L Cells,Strain L Cells,Cell Line L-Cell,Cell Line L-Cells,Cell Line, L,Cell Line, L929,Cell Lines, L,Cell, L,Cell, L (Cell Line),Cell, L929,Cell, Strain L,Cells, L,Cells, L (Cell Line),Cells, L929,Cells, Strain L,L Cell,L Cell (Cell Line),L Cell Lines,L Cell, Strain,L Cells, Cell Line,L Cells, Strain,L-Cell,L-Cell Lines,L-Cell, Cell Line,L929 Cell,Strain L Cell
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002691	Chlamydophila psittaci	A genus of CHLAMYDOPHILA infecting primarily birds. It contains eight known serovars, some of which infect more than one type of host, including humans.	Chlamydia psittaci
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining