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Preparations of human glomerular basement membrane (GBM) were digested with collagenase, and a Goodpasture (GP) antigen rich pool from gel filtration column runs was identified by antibody inhibition radioimmunoassay. The components of the GP antigen pool were separated on polyacrylamide gels, and transferred to nitrocellulose sheets by the 'western' blotting technique. The blots were separately reacted with thirteen GP sera as primary antibody, followed by peroxidase labelled goat anti-human IgG and revealed 45-50K (two bands) and 25-28K (one-three bands) components. No corresponding reactivity was observed using convalescent GP sera or other control sera (normal human serum, rapidly progressive glomerulonephritis with or without pulmonary haemorrhage, and lupus erythematosus) as primary antibody.
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
March 1984,
Proceedings of the National Academy of Sciences of the United States of America,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
August 1973,
Archives of internal medicine,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
April 2023,
Clinical and experimental rheumatology,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
March 1990,
Clinical nephrology,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
June 1984,
Proceedings of the National Academy of Sciences of the United States of America,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
February 1982,
Biochemical and biophysical research communications,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
June 2020,
Proceedings (Baylor University. Medical Center),
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
September 1989,
Laboratory investigation; a journal of technical methods and pathology,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
February 2022,
Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy,
A J Fish, and
M C Lockwood, and
M Wong, and
R G Price
December 1988,
Clinical and experimental immunology,
