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A cDNA clone containing the complete human alpha 1-antitrypsin sequence was isolated from a human liver cDNA bank by screening with a chemically synthesized oligonucleotide probe. DNA sequences encoding the alpha 1-antitrypsin mature polypeptide were inserted into an Escherichia coli expression vector that allows transcription from the efficient leftward promoter of bacteriophage lambda (PL) and initiation of translation at the lambda cII gene ribosome-binding site. This construction resulted in the induction of a 45-kilodalton protein at a level of approximately 15% of total cell protein. The polypeptide produced was recognized by antisera raised against human alpha 1-antitrypsin protein and displayed normal biological activity in an in vitro antielastase assay.
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
October 1987,
Molecular biology & medicine,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
July 1990,
Proceedings of the National Academy of Sciences of the United States of America,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
November 2018,
Protein expression and purification,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
November 1995,
Gene,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
December 1990,
Gene,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
December 1992,
Gene,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
January 1984,
FEBS letters,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
October 1991,
Molecular and cellular endocrinology,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
January 1992,
International journal of peptide and protein research,
M Courtney, and
A Buchwalder, and
L H Tessier, and
M Jaye, and
A Benavente, and
A Balland, and
V Kohli, and
R Lathe, and
P Tolstoshev, and
J P Lecocq
January 2017,
Methods in molecular biology (Clifton, N.J.),
