Biomaterial Database

Catalytic activity of neutrophil and eosinophil peroxidase. Cytochemical and biochemical study in total hereditary myeloperoxidase deficiency. 1984

U Lippi, and P Cappelletti, and M Schinella

We studied myeloperoxidase and eosinophil peroxidase activity in healthy subjects and in four totally myeloperoxidase deficient subjects using cytochemical and biochemical methods. Cytochemical tests demonstrated a pH dependent, enzymatic differentiated affinity of myeloperoxidase and eosinophil peroxidase for the chromogens used. The eosinophil peroxidase/myeloperoxidase ratio varied from 1.5 to 4. Biochemical assays, using MBTH-AA or o-dianisidine methods, produced eosinophil peroxidase/myeloperoxidase ratios of 1.98 and 1.72.

UI	MeSH Term	Description	Entries
D009195	Peroxidase	A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.	Myeloperoxidase,Hemi-Myeloperoxidase,Hemi Myeloperoxidase
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D010544	Peroxidases		Ovoperoxidase
D004804	Eosinophils	Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.	Eosinophil
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations