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The binding of [3H]prazosin and [3H]clonidine to rat jejunal epithelial cell membranes has been studied. The membrane preparation was enriched in baso-lateral components as determined by Na+, K+ ATPase and alkaline phosphatase activities. The membranes possessed two saturable specific binding sites for [3H]prazosin, a high affinity (Kd 0.17 nM) low capacity (Bmax 27.3 fmole bound per mg protein) and a low affinity (Kd 5.0 nM) high capacity (Bmax 276 fmole bound per mg protein) site. The specificity of both sites was similar and was related to alpha 1-adrenoceptors. [3H]Clonidine bound to the membranes in a saturable fashion (Kd 7.3 nM). The specificity of this site was related to alpha 2-adrenoceptors. The [3H]clonidine binding site was present in the membranes in much lower density (Bmax 22.8 fmole bound per mg protein) suggesting that alpha 1-adrenoceptors predominate in this tissue.
D J Cotterell, and
K A Munday, and
J A Poat
March 1981,
The Journal of pharmacy and pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
February 1982,
Neurochemical research,
D J Cotterell, and
K A Munday, and
J A Poat
November 1986,
Research communications in chemical pathology and pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
June 1983,
Japanese journal of pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
May 1982,
European journal of pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
February 1984,
European journal of pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
August 1980,
European journal of pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
February 1986,
Biochemical pharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
November 1984,
Neuropharmacology,
D J Cotterell, and
K A Munday, and
J A Poat
May 1981,
British journal of pharmacology,
